Journal article
Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale
S Kho, NM Anstey, BE Barber, K Piera, T William, E Kenangalem, JS McCarthy, IK Jang, GJ Domingo, S Britton, MJ Grigg
Scientific Reports | NATURE PORTFOLIO | Published : 2022
Abstract
Commercial point-of-care tests remain insufficient for accurately detecting and differentiating low-level malaria infections in regions co-endemic with multiple non-falciparum species, including zoonotic Plasmodium knowlesi (Pk). A 5-plex chemiluminescent assay simultaneously measures pan-Plasmodium lactate dehydrogenase (pLDH), P. falciparum (Pf)-LDH, P. vivax (Pv)-LDH, Pf-histidine-rich protein-2 (HRP2), and C-reactive protein. We assessed its diagnostic performance on whole blood (WB) samples from 102 healthy controls and 306 PCR-confirmed clinical cases of Pf, Pv, Pk, P. malariae (Pm) and P. ovale (Po) mono-infections from Southeast-Asia. We confirm its excellent HRP2-based detection of ..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
Funding support from the Australian National Health and Medical Research Council (the Australian Centre of Research Excellence in Malaria Elimination, Program Grant #1,037,304, and Fellowships to SK [Improving Health Outcomes in the Tropical North (HOTNORTH); #1131932], NMA [#1135820] and MJG [#1138860]). MJG is supported by the Australian Centre for International Agricultural Research (Grant #LS2019116). GJD and IKJ are supported by the Bill & Melinda Gates Foundation (Grant # INV1135840). We thank the Director-General, Ministry of Health, Malaysia for permission to publish this paper.